Determinants of Human Milk Donation and Use among Postpartum Women at a City Hospital Nairobi, KenyaBackground: World Health Organization advocates donor human milk as a superior substitute for newborns unable to obtain mother’s milk. Calls have been made for the scale-up of human milk banks to provide infants without access to mother’s milk safe donor human milk.Research aim: To assess determinants of human milk donation and use among postpartum women at a city hospital with human milk banking facilities.Methods: This was a cross-sectional study. Consecutive sampling method was used to recruit three hundred and seventy mothers. Data was collected using a pre-tested interview-based questionnaire. Data was analyzed using R-4.3.0 software and descriptive statistics were done for all variables. Further inferential statistics including Chi-Square test and multivariate logistics regression were computed with significance level set at α=0.05.Results: The mean age of the participants was 27 years (±6.3). A majority (78.6%) of the women were married and slightly above half (57%) had attained secondary school education. Only 27.3% were aware of human milk banking. Mother’s willingness to donate human milk and use donor human milk was 78.1% and 70.8% respectively. The respondent’s age (OR 0.423; 95% CI 0.19-0.942) was significantly associated with willingness to donate. Religion (OR 0.266; 95% CI 0.097-0.718) and prior knowledge of human milk banking (OR 0.894; 95% CI 1.066-3.364) were significant predictors of willingness to use donor human milk.Conclusion: Human milk banking awareness was low among the study population; however, mothers are willing to donate human milk as well as feed their infants’ donor human milk.