Stigma surrounding mental health persists as a significant impediment to adequate support and help-seeking behaviors in Slovenia, despite advancements in psychiatric care and education. This study investigates the prevalence of stigma among first-year medical students at the University of Maribor, Slovenia who lack prior exposure to mental health education. A total of 118 students participated in the baseline assessment, with 66 students completing the questionnaire post-intervention. The intervention involved implementing the Life Transitions program, aimed at enhancing mental health literacy and reducing stigma. Ther was a significant reduction in stigma after intervention (Wilcoxon Z= -3.06, p<0.01), suggesting a positive impact of the educational program. This reduction aligns with findings from similar programs globally, suggesting the efficacy of educational interventions in mitigating mental health stigma. The study emphasizes the importance of integrating mental health education into medical curricula to foster stigma reduction and enhance mental health literacy among future healthcare professionals.