ITS region
The phylogenetic analyses based on 29 ITS sequences are shown in Figure
1A (NJ), 1B (MP), and 1C (ML). The generated trees show that the
analysed Tulipa taxa divided into two main clades. The first
clade includes specimens of the subgenus Eriostemones (T.
sylvestris including both subspecies) strongly supported by NJ (100%
BS), MP (100% BS) and ML (95%), while the second clade includes
members of the subgenus Tulipa (T. albanica, T. kosovarica,
T. luanica, T. scardica, T. serbica, T. ulophylla, T. tschimganica, T.
suaveolens, T. julia and T. gesneriana ) 100% supported by all
three calculation methods. In the first clade, the newly
sequenced samples of T. sylvestris , T. sylvestrissubsp. sylvestris and T. sylvestris subsp.
australis separated from T. sylvestris subsp. sylvestrisprovided from the GenBank. In the second clade, the newly sequenced
species of T. albanica, T. kosovarica, T. luanica, T. scardicaand T. serbica are grouped with already published sequences ofT. ulophylla, T. tschimganica, T. suaveolens, T. julia, andT. gesneriana . Within the Tulipa clade, in all trees the
most distinct species from the newly generate sequences was T.
tschimganica (section Spiranthera ), followed by T. julia,
T. ulophylla (both section Tulipanum ) and T. suaveolensand T. gesneriana , which were the closest related to newly
sequenced species (all section Tulipa ). The separation ofT. suaveolens and T. gesneriana from newly sequenced
species (T. albanica, T. kosovarica, T. luanica, T. scardica, T.
serbica ) in general was strongly supported by NJ (97%) and moderately
supported by MP (86%) and ML (78%). Species specific grouping of the
newly sequenced samples was not supported in all trees, except T.
albanica which was moderately supported only in NJ (82%). A clade
including all samples of T. albanica and one sample of T.
kosovarica (T6), T. luanica (T13) and T. serbica (T19)
respectively, was weakly supported in NJ (less than 54%) and MP (less
than 64%).
Figure 1. Phylogenetic tree constructed from ITS dataset of theTulipa taxa.