Combined ITS + trnL-trnF dataset
The phylogenetic trees obtained from the combined ITS andtrnL-trnF dataset are shown in 3A (NJ), 3B (MP) and 3C (ML). The generated trees show that the analysed Tulipa species are divided into two main clades too. The first clade (specimens of subgenusEriostemones ) is strongly supported in all trees (99-100%) and the second clade (specimens of subgenus Tulipa ) is strongly supported (97-100%) in all trees too. The generated trees from the combined dataset are more similar with trees obtained from ITS sequences only, but in general with stronger support. In the Tulipa clade, the species T. tschimganica (section Spiranthera ), was the most distant species from the newly sequenced species (T. albanica, T. kosovarica, T. luanica, T. scardica and T. serbica ) moderately to strongly supported in all trees, followed by T. julia and T. ulophylla (section Tulipanum ) moderately supported by NJ (76%) and by MP (76%), but not supported by ML (<50%). The specimen of T. suaveolens was the closest related to the newly sequenced species, as all of these species belongs to section Tulipa . Within the newly sequenced species T. albanica separated from T. scardica, T. serbica, T. kosovaricaand T. luanica within the NJ (87%) and MP (85%) trees. The other specimens showed slightly intra-specific variation (T. kosovarica samples T6 and T7, weakly supported by NJ (65%), MP (64%) and ML (65%)) but no species specific grouping.
Figure 3. Phylogenetic tree constructed from ITS +trnL-trnF data of the Tulipa taxa.