trnL-trnF region
Plastid data obtained from trnL-trnF sequences shown in Figure 2A (NJ), 2B (MP) and 2C (ML) resemble those found in the ITS sequences analysis, but in general with lower resolution. The trees obtained from analysed sequences are divided into two major clades too. The first clade consists of members of the subgenus Eriostemones (T. sylvestris including both subspecies), which is moderately supported by NJ (86%) and ML (74%) but not supported by MP (<50%). In the Eriostemones clade, the published sequence of T. sylvestris subsp. sylvestrisshowed sequence differences to the newly obtained sequences of the species. The second clade consists of members of the subgenusTulipa, belonging to section Tulipa (T. albanica, T. kosovarica, T. luanica, T. scardica, T. serbica, T. suaveolens andT. gesneriana ), section Tulipanum (T. julia andT. ulophylla ) and section Spiranthera (T. tschimganica ). The subgenus Tulipa is moderately supported by NJ (87%), MP (89%) and ML (89%). Furthermore, the specimens of subgenus Tulipa divides into two subclades, the first subclade consists of T. kosovarica, T. Luanica and T. serbica, which was moderately supported by NJ (86%) but not supported by MP and ML, while the second subclade consisting of T. albanica, T. scardica, T. julia, T. ulophylla, T. Suaveolens and T. tschimganica was moderately supported by NJ (84%) and ML (76%), but not supported by MP. Within the first subclade, in all phylogenetic trees T. kosovarika (samples T6, T7 and T8) were further divided with weak support by NJ (63%), ML (62%) and MP (67%).
Figure 2. Phylogenetic tree constructed from trnL-trnFdataset of the Tulipa taxa.