To achieve more accurate and robust Differential Item Functioning (DIF) detection, this study introduces a novel approach called Multi-Detectors Combination (MDC). The main innovation of the MDC approach lies in effectively integrating multiple existing single DIF detection methods under observable examination conditions. Four specific MDC methods (LR, NB, TAN, and SVM) are evaluated using simulated data (360 testing conditions). The results indicate that MDC methods outperform single DIF detection in accuracy (AUC: 81.2% to 82.3%), surpassing the AUC values of other methods, which ranged only from 59.4% to 77.4%. Moreover, MDC methods demonstrate enhanced precision (RMSE: 0.528 to 0.531) in ability estimation. Analysis of actual data further validates the MDC approach’s superior stability in ability estimation, achieved by excluding flagged DIF items.