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A Pilot Study of Plasma Microbial Cell-Free DNA Following Chimeric Antigen Receptor T Cell Therapy in Pediatric Patients with Relapsed/Refractory Leukemia
  • +3
  • Catherine Aftandilian,
  • Xue Rachel Bito,
  • David Berman,
  • Amy Zhang,
  • Radha Duttagupta,
  • Kara Davis
Catherine Aftandilian
Stanford University

Corresponding Author:[email protected]

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Xue Rachel Bito
Children's Hospital Los Angeles Children's Center for Cancer and Blood Diseases
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David Berman
Karius Inc
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Amy Zhang
Stanford University Department of Medicine
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Radha Duttagupta
Nucleix Ltd
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Kara Davis
Stanford University
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Abstract

Chimeric antigen receptor (CAR) T cell therapy is a promising treatment for pediatric patients with relapsed or refractory B cell acute lymphoblastic leukemia (R/R B ALL). Cytokine release syndrome (CRS) is a common toxicity after CAR T cell therapy and fever is often the first symptom. Differentiating CRS from infection after CAR T cell therapy can be challenging. Plasma microbial cell free DNA (mcfDNA) is a novel diagnostic tool which allows for qualitative and quantitative assessment of over 1,000 organisms. This pilot study sought to characterize mcfDNA results in pediatric patients with R/R B ALL in the first two months after CAR T cell therapy.