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The purpose of the study was to analyze the role of spirituality and religiosity for the well-being of transgender people in Poland. Spirituality understood as a personality construct referring to the natural human’s pursuit of transcendence was included in the study as a component of three indicators: religious spirituality, ethical sensitivity, and harmony, where religiosity was defined as interest in religious issues, beliefs, prayer, experience, and worship. It was assumed in the study that happiness, as one of the explanations of human well-being, is related to positive emotions, optimal experiences, and the meaning of life. The study involved 141 transgender persons (90 trans-men and 51 trans-women) aged 14 to 57. The results indicate a strong relation between spirituality, and weak relationship between religiosity and felt happiness. At the same time, it was not found that spirituality influenced the level of happiness depending on the level of religiosity and differences in spirituality due to religion.