The cognitive abilities that are necessary to make-mating interactions effectively have been described as “Mating Intelligence.” This is an evolutionary construct that has just recently initiated to receive practical consideration. This research work investigated the psychometric properties of the Persian translation of the Mating Intelligence Scale in Iran. A total of 760 male and female heterosexual participants (380 for each sex) were recruited from colleges. Participants completed a self-reported questionnaire and reported some demographic information such as age, their qualification degree and major. The male and female version of the Geher and Kaufman (2007) Mating Intelligence Scale were used to gather data. The factor analysis with the Oblimin rotation showed five factors with Eigen-values greater than one (Sexual Over-Estimation, Cross-Sex Mind Reading, Self-Deception, Other-Deception, and Courtship Display) in males sample. Also, a confirmatory factor analysis was performed to confirm the results of the exploratory analysis. The values of fitness indicators show that this model benefits from good fitness. Results showed four factors with eigenvalues greater than one (Sexual Over-Estimation, Cross-Sex Mind Reading, Other-Deception, and Courtship Display) in females sample, and again, the confirmatory factor analysis confirmed the results of exploratory analysis. In general, the use of the MI questionnaire is appropriate regarding the Iranian population due to the excellent validity and reliability and can be used in screening, therapeutic and educational situations.